Scanlon, M. J., Ohtsu, K., Timmermans, M. C. P., Schnable, P. S. (July 2009) Laser microdissection-mediated isolation and in vitro transcriptional amplification of plant RNA. Current Protocols in Molecular Biology (SUPPL.). 25A.3.1-25A.3.15.
Abstract
Protocols for laser microdissection and linear amplification of RNA from fixed, sectioned plant tissues are described. When combined with quantitative RT-PCR, microarray analysis, or RNA-sequencing, these procedures enable quantitative analyses of transcript accumulation from microscopic quantities of specific plant organs, tissues, or single cells. © 2009 by John Wiley & Sons, Inc.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Laser microdissection Plants RNA amplification Transcriptomics |
| Subjects: | bioinformatics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics Investigative techniques and equipment bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA expression organism description > plant |
| CSHL Authors: | |
| Communities: | CSHL labs > Timmermans lab |
| Depositing User: | Matt Covey |
| Date: | July 2009 |
| Date Deposited: | 20 Feb 2013 22:06 |
| Last Modified: | 20 Feb 2013 22:06 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/27403 |
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