DOCK7 interacts with TACC3 to regulate interkinetic nuclear migration and cortical neurogenesis

Yang, Y. T., Wang, C. L., Van Aelst, L. (August 2012) DOCK7 interacts with TACC3 to regulate interkinetic nuclear migration and cortical neurogenesis. Nature Neuroscience, 15 (9). pp. 1201-10. ISSN 1097-6256

URL: http://www.ncbi.nlm.nih.gov/pubmed/22842144

DOI: 10.1038/nn.3171

Abstract

Neurogenesis in the developing neocortex relies on the ability of radial glial progenitor cells (RGCs) to switch from proliferative to differentiative neuron-generating divisions, but the molecular mechanisms that control this switch in a correct temporal manner are not well understood. Here, we show that DOCK7, a member of the DOCK180 family of proteins, regulates RGC proliferation versus differentiation. Silencing of DOCK7 in RGCs of developing mouse embryos impedes neuronal differentiation and maintains cells as cycling progenitors. In contrast, DOCK7 overexpression promotes RGC differentiation to basal progenitors and neurons. We further present evidence that DOCK7 influences neurogenesis by controlling apically directed interkinetic nuclear migration of RGCs. DOCK7 exerts its effects by antagonizing the microtubule growth-promoting function of the centrosome-associated protein TACC3. Thus, DOCK7 interaction with TACC3 controls interkinetic nuclear migration and the genesis of neurons from RGCs during cortical development.

Item Type:	Paper
Subjects:	organs, tissues, organelles, cell types and functions > organs types and functions > brain organs, tissues, organelles, cell types and functions > cell types and functions organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > organs types and functions organs, tissues, organelles, cell types and functions
CSHL Authors:	Van Aelst, Linda Wang, Chia-Lin
Communities:	CSHL Cancer Center Shared Resources > Animal Services CSHL Cancer Center Shared Resources > DNA Sequencing Service CSHL Cancer Center Shared Resources > Microscopy Service CSHL Post Doctoral Fellows CSHL labs > Van Aelst lab CSHL Cancer Center Program > Signal Transduction
Depositing User:	Matt Covey
Date:	12 August 2012
Date Deposited:	17 Jan 2013 20:32
Last Modified:	16 Oct 2015 15:39
PMCID:	PMC3431462
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/27087

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