Genetic Framework of Cyclin-Dependent Kinase Function in Arabidopsis

Nowack, Moritz K., Harashima, Hirofumi, Dissmeyer, Nico, Zhao, Xin'Ai, Bouyer, Daniel, Weimer, Annika K., De Winter, Freya, Yang, Fang, Schnittger, Arp (May 2012) Genetic Framework of Cyclin-Dependent Kinase Function in Arabidopsis. Developmental Cell, 22 (5). pp. 1030-1040. ISSN 1534-5807

Abstract

Summary Cyclin-dependent kinases (CDKs) are at the heart of eukaryotic cell-cycle control. The yeast Cdc2/CDC28 PSTAIRE kinase and its orthologs such as the mammalian Cdk1 have been found to be indispensable for cell-cycle progression in all eukaryotes investigated so far. CDKA;1 is the only PSTAIRE kinase in the flowering plant Arabidopsis and can rescue Cdc2/CDC28 mutants. Here, we show that cdka;1 null mutants are viable but display specific cell-cycle and developmental defects, e.g., in S phase entry and stem cell maintenance. We unravel that the crucial function of CDKA;1 is the control of the plant Retinoblastoma homolog RBR1 and that codepletion of RBR1 and CDKA;1 rescued most defects of cdka;1 mutants. Our work further revealed a basic cell-cycle control system relying on two plant-specific B1-type CDKs, and the triple cdk mutants displayed an early germline arrest. Taken together, our data indicate divergent functional differentiation of Cdc2-type kinases during eukaryote evolution.

Item Type:	Paper
Subjects:	organism description > plant > Arabidopsis bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase organism description > plant bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
CSHL Authors:	Yang, Fang
Communities:	CSHL labs > Jackson lab CSHL Post Doctoral Fellows
Depositing User:	Matt Covey
Date:	15 May 2012
Date Deposited:	23 Jan 2013 22:05
Last Modified:	03 May 2013 15:52
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/27014

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