Helmstaedter, M., Mitra, P. P.
(February 2012)
Computational methods and challenges for large-scale circuit mapping.
Current Opinion in Neurobiology, 22 (1).
pp. 162-169.
ISSN 09594388 (ISSN)
Abstract
The connectivity architecture of neuronal circuits is essential to understand how brains work, yet our knowledge about the neuronal wiring diagrams remains limited and partial. Technical breakthroughs in labeling and imaging methods starting more than a century ago have advanced knowledge in the field. However, the volume of data associated with imaging a whole brain or a significant fraction thereof, with electron or light microscopy, has only recently become amenable to digital storage and analysis. A mouse brain imaged at light-microscopic resolution is about a terabyte of data, and 1mm 3 of the brain at EM resolution is about half a petabyte. This has given rise to a new field of research, computational analysis of large-scale neuroanatomical data sets, with goals that include reconstructions of the morphology of individual neurons as well as entire circuits. The problems encountered include large data management, segmentation and 3D reconstruction, computational geometry and workflow management allowing for hybrid approaches combining manual and algorithmic processing. Here we review this growing field of neuronal data analysis with emphasis on reconstructing neurons from EM data cubes. © 2012.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
algorithm
anisotropy
brain mapping
computer analysis
confocal microscopy
data analysis
geometry
image processing
image reconstruction
nerve cell network
neuroanatomy
neurobiology
neuroimaging
nonhuman
perikaryon
priority journal
process optimization
reliability
review
scanning electron microscopy
three dimensional imaging
transmission electron microscopy |
| Subjects: |
organs, tissues, organelles, cell types and functions > organs types and functions > brain
organs, tissues, organelles, cell types and functions > cell types and functions > cell types
organs, tissues, organelles, cell types and functions > cell types and functions > cell types
organs, tissues, organelles, cell types and functions > cell types and functions > cell types
organs, tissues, organelles, cell types and functions > cell types and functions
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > neuronal circuits
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > neuronal circuits
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > neuronal circuits
organs, tissues, organelles, cell types and functions > organs types and functions
organs, tissues, organelles, cell types and functions |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Mitra lab |
| Depositing User: |
Matt Covey
|
| Date: |
February 2012 |
| Date Deposited: |
30 Jan 2013 20:34 |
| Last Modified: |
30 Jan 2013 20:34 |
| PMCID: |
PMC3406305 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/26957 |
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