Hannon, G. J., Chubb, A., Maroney, P. A., Hannon, G., Altman, S., Nilsen, T. W. (1991) Multiple cis-acting elements are required for RNA polymerase III transcription of the gene encoding H1 RNA, the RNA component of human RNase P. Journal of Biological Chemistry, 266 (34). pp. 22796-22799. ISSN 00219258 (ISSN)
Abstract
In humans, the H1 RNA, the RNA subunit of RNase P, is synthesized by RNA polymerase III. We have used block replacement mutagenesis to identify the sequences necessary for in vitro transcription of H1 RNA. We find that multiple cis-acting elements located in the H1 RNA 5'-flanking region are necessary for H1 RNA synthesis; no internal sequences are essential. Required cis-acting elements include sequences resembling proximal sequence element, distal sequence element, and TATA motifs. In this respect, the H1 RNA promoter is similar in structure to the promoters of the genes encoding the U6 snRNA, the 7 SK RNA and the MRP RNA. However, our mutational analysis indicates that the H1 promoter is unexpectedly complex, with several additional cis-acting elements spanning nearly 70 base pairs of the H1 RNA gene 5'-flanking sequence.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | dna directed rna polymerase iii ribonuclease p article genetic transcription human priority journal promoter region site directed mutagenesis structure activity relation tata box Base Sequence DNA Endoribonucleases Gene Expression Regulation, Enzymologic Molecular Sequence Data Mutagenesis, Site-Directed Promoter Regions (Genetics) RNA RNA Polymerase III RNA, Catalytic Support, U.S. Gov't, Non-P.H.S. Support, U.S. Gov't, P.H.S. Templates, Genetic Transcription, Genetic |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > RNA polymerase bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > RNA binding protein |
| CSHL Authors: | |
| Communities: | CSHL labs > Hannon lab |
| Depositing User: | Matt Covey |
| Date: | 1991 |
| Date Deposited: | 09 Jan 2013 17:35 |
| Last Modified: | 09 Jan 2013 17:35 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/26500 |
Actions (login required)
 |
Administrator's edit/view item |