Hannon, G. J., Maroney, P. A., Yu, Y. T., Hannon, G. E., Nilsen, T. W. (1992) Interaction of U6 snRNA with a sequence required for function of the nematode SL RNA in trans-splicing. Science, 258 (5089). pp. 1775-1780. ISSN 00368075 (ISSN)
Abstract
Nematode trans-spliced leader (SL) RNAs are composed of two domains, an exon [the 22-nucleotide spliced leader] and a small nuclear RNA (snRNA)-like sequence. Participation in vitro of the spliced leader RNA in trans-splicing reactions is independent of the exon sequence or size and instead depends on features contained in the snRNA-like domain of the molecule. Chemical modification interference analysis has revealed that two short sequence elements in the snRNA-like domain are necessary for SL RNA activity. These elements are sufficient for such activity because when added to a 72-nucleotide fragment of a nematode U1 snRNA, this hybrid RNA could participate in trans-splicing reactions in vitro. One of the critical sequence elements may function by base-pairing with U6 snRNA, an essential U snRNA for both cis- and trans-splicing.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | rna small nuclear rna article molecular interaction nematode nonhuman priority journal rna splicing Animal Ascaris Base Sequence Binding Sites Exons Models, Structural Molecular Sequence Data Nucleic Acid Conformation RNA, Messenger RNA, Small Nuclear Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA splicing bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > splicing factor |
| CSHL Authors: | |
| Communities: | CSHL labs > Hannon lab |
| Depositing User: | Matt Covey |
| Date: | 1992 |
| Date Deposited: | 09 Jan 2013 17:36 |
| Last Modified: | 09 Jan 2013 17:36 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/26497 |
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