Transformation of normal human cells in the absence of telomerase activation

Seger, Y. R., García-Cao, M., Piccinin, S., Lo Cunsolo, C., Doglioni, C., Blasco, M. A., Hannon, G. J., Maestro, R. (2002) Transformation of normal human cells in the absence of telomerase activation. Cancer Cell, 2 (5). pp. 401-413. ISSN 15356108 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/12450795

DOI: 10.1016/s1535-6108(02)00183-6

Abstract

Our knowledge of the transformation process has emerged largely from studies of primary rodent cells and animal models. However, numerous attempts to transform human cells using oncogene combinations that are effective in rodents have proven unsuccessful. These findings strongly argue for the study of homologous experimental systems. Here we report that the combined expression of adenovirus E1A, Ha-RasV12, and MDM2 is sufficient to convert a normal human cell into a cancer cell. Notably, transformation did not require telomerase activation. Therefore, we provide evidence that activation of telomere maintenance strategies is not an obligate characteristic of tumorigenic human cells. Copyright © 2002 Cell Press.

Item Type:	Paper
Uncontrolled Keywords:	adenovirus vector E1A protein protein MDM2 Ras protein telomerase animal cell animal experiment animal model animal tissue article cancer cell carcinogenesis cell transformation controlled study enzyme activation human human cell mouse nonhuman priority journal telomere virus expression animal cell culture cell transplantation chromosome aberration comparative study fibroblast genetics metabolism nude mouse physiology Retrovirus virus cell transformation Adenoviridae Animalia Rodentia Animals Cell Transformation, Neoplastic Cell Transformation, Viral Cells, Cultured Chromosome Aberrations Fibroblasts Humans Mice Mice, Nude Retroviridae
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes organism description > animal > mammal > primates > hominids organism description > animal > mammal > primates > hominids > human organism description > animal > mammal organism description > animal > mammal > primates bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > telomerase
CSHL Authors:	Hannon, Gregory J. Seger, Yvette R.
Communities:	CSHL labs > Hannon lab
Depositing User:	Matt Covey
Date:	2002
Date Deposited:	09 Jan 2013 16:57
Last Modified:	09 Jan 2013 16:57
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/26463

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