Bernstein, E., Kim, S. Y., Carmell, M. A., Murchison, E. P., Alcorn, H., Li, M. Z., Mills, A. A., Elledge, S. J., Anderson, K. V., Hannon, G. J.
(2003)
Dicer is essential for mouse development.
Nature Genetics, 35 (3).
pp. 215-217.
ISSN 10614036 (ISSN)
Abstract
To address the biological function of RNA interference (RNAi)-related pathways in mammals, we disrupted the gene Dicer1 in mice. Loss of Dicer1 lead to lethality early in development, with Dicer1-null embryos depleted of stem cells. Coupled with our inability to generate viable Dicer1-null embryonic stem (ES) cells, this suggests a role for Dicer, and, by implication, the RNAi machinery, in maintaining the stem cell population during early mouse development.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
RNA
animal cell
animal model
article
cell viability
dicer 1 gene
embryo
embryo development
gene
gene disruption
gene function
gene silencing
lethality
mammal
mouse
nonhuman
priority journal
stem cell
Amino Acid Sequence
Animals
DEAD-box RNA Helicases
Embryonic and Fetal Development
Endoribonucleases
Mice
Mice, Knockout
Molecular Sequence Data
RNA Helicases
RNA Interference
Stem Cells
Animalia
Mammalia |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > dicer
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > dicer
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes
organism description > animal > mammal
organism description > animal > mammal > rodent > mouse
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
organism description > animal > mammal > rodent |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Hannon lab
School of Biological Sciences > Publications |
| Depositing User: |
Matt Covey
|
| Date: |
2003 |
| Date Deposited: |
09 Jan 2013 16:58 |
| Last Modified: |
19 Sep 2014 14:34 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/26461 |
Actions (login required)
 |
Administrator's edit/view item |
