Nicholson, D. W., Ali, A., Thornberry, N. A., Vaillancourt, J. P., Ding, C. K., Gallant, M., Gareau, Y., Griffin, P. R., Labelle, M., Lazebnik, Y. A. (1995) Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis. Nature, 376 (6535). pp. 37-43. ISSN 0028-0836
Abstract
The protease responsible for the cleavage of poly(ADP-ribose) polymerase and necessary for apoptosis has been purified and characterized. This enzyme, named apopain, is composed of two subunits of relative molecular mass (M(r)) 17K and 12K that are derived from a common proenzyme identified as CPP32. This proenzyme is related to interleukin-1 beta-converting enzyme (ICE) and CED-3, the product of a gene required for programmed cell death in Caenorhabditis elegans. A potent peptide aldehyde inhibitor has been developed and shown to prevent apoptotic events in vitro, suggesting that apopain/CPP32 is important for the initiation of apoptotic cell death.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amino Acid Sequence Animals Apoptosis Caenorhabditis elegans Caenorhabditis elegans Proteins Caspase 1 Caspase 3 Caspases Cysteine Endopeptidases Cysteine Proteinase Inhibitors Enzyme Precursors Helminth Proteins Humans Kinetics Mass Spectrometry Molecular Sequence Data Poly(ADP-ribose) Polymerases Tumor Cells, Cultured |
| Subjects: | organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > apoptosis organs, tissues, organelles, cell types and functions > cell types and functions > cell functions organs, tissues, organelles, cell types and functions > cell types and functions |
| CSHL Authors: | |
| Communities: | CSHL labs > Labeznik lab |
| Depositing User: | Matt Covey |
| Date: | 1995 |
| Date Deposited: | 12 Dec 2012 17:32 |
| Last Modified: | 12 Dec 2012 17:32 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/26402 |
Actions (login required)
 |
Administrator's edit/view item |