Lassus, Patrice, Opitz-Araya, Ximena, Lazebnik, Yuri (2002) Requirement for caspase-2 in stress-induced apoptosis before mitochondrial permeabilization. Science, 297 (5585). pp. 1352-1354. ISSN 1095-9203
Abstract
A current view is that cytotoxic stress, such as DNA damage, induces apoptosis by regulating the permeability of mitochondria. Mitochondria sequester several proteins that, if released, kill by activating caspases, the proteases that disassemble the cell. Cytokines activate caspases in a different way, by assembling receptor complexes that activate caspases directly; in this case, the subsequent mitochondrial permeabilization accelerates cell disassembly by amplifying caspase activity. We found that cytotoxic stress causes activation of caspase-2, and that this caspase is required for the permeabilization of mitochondria. Therefore, we argue that cytokine-induced and stress-induced apoptosis act through conceptually similar pathways in which mitochondria are amplifiers of caspase activity rather than initiators of caspase activation.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Apoptosis Apoptotic Protease-Activating Factor 1 Caspase 2 Caspases Cell Line, Transformed Cytochrome c Group DNA Damage Enzyme Activation Enzyme Repression Etoposide Humans Mitochondria Permeability Protein Transport Proteins Proto-Oncogene Proteins Proto-Oncogene Proteins c-bcl-2 RNA, Small Interfering RNA, Untranslated Signal Transduction Transfection Tumor Cells, Cultured Tumor Necrosis Factor-alpha bcl-2-Associated X Protein |
| Subjects: | organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > apoptosis organs, tissues, organelles, cell types and functions > cell types and functions > cell functions organs, tissues, organelles, cell types and functions > organelles, types and functions > mitochondria organs, tissues, organelles, cell types and functions > organelles, types and functions organs, tissues, organelles, cell types and functions |
| CSHL Authors: | |
| Communities: | CSHL labs > Labeznik lab |
| Depositing User: | Matt Covey |
| Date: | 2002 |
| Date Deposited: | 12 Dec 2012 17:29 |
| Last Modified: | 12 Dec 2012 17:29 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/26383 |
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