Immunofluorescence localization of nuclear proteins

Spector, David L. (2011) Immunofluorescence localization of nuclear proteins. Cold Spring Harbor Protocols, 2011 (10). pp. 1276-1280. ISSN 1559-6095

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Abstract

Many nuclear proteins have been successfully localized using immunofluorescence microscopy. These proteins span all nuclear domains, including the nuclear envelope, nuclear lamina, nucleolus, chromatin-associated proteins, and proteins associated with RNA metabolism and nuclear bodies. This article describes a general method for localizing nuclear proteins. Cells grown on coverslips are fixed in either formaldehyde or methanol and permeabilized in Triton X-100. Incubating the cells with primary antibody and fluorescently conjugated secondary antibody allows visualization of the target antigen.

Item Type:	Paper
Uncontrolled Keywords:	Cells, Cultured Fluorescent Antibody Technique Formaldehyde Methanol Nuclear Proteins Octoxynol Surface-Active Agents
Subjects:	Investigative techniques and equipment Investigative techniques and equipment > microscopy > flourescence microscopy Investigative techniques and equipment > microscopy > immunoflourescence microscopy Investigative techniques and equipment > microscopy
CSHL Authors:	Spector, David L.
Communities:	CSHL labs > Spector lab
Depositing User:	Matt Covey
Date:	2011
Date Deposited:	10 Dec 2012 20:02
Last Modified:	29 Jan 2015 21:12
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/26373

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