Muratani, M., Gerlich, D., Janicki, S. M., Gebhard, M., Eils, R., Spector, D. L.
(2002)
Metabolic-energy-dependent movement of PML bodies within the mammalian cell nucleus.
Nature Cell Biology, 4.
pp. 106-110.
ISSN 1465-7392
Abstract
Promyelocytic leukaemia (PML) nuclear bodies are present in most mammalian cell nuclei. PML bodies are disrupted by PML retinoic acid receptor alpha (RAR alpha) oncoproteins in acute promyelocytic leukaemia. These bodies contain numerous proteins, including Sp100, SUMO-1, HAUSP(USP7), CBP and BLM, and they have been implicated in aspects of transcriptional regulation or as nuclear storage depots. Here, we show that three classes of PML nuclear bodies can be distinguished, on the basis of their dynamic properties in living cells. One class of PML bodies is particularly noteworthy in that it moves by a metabolic-energy-dependent mechanism. This represents the first example of metabolic-energy-dependent transport of a nuclear body within the mammalian cell nucleus.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
Animals
Antigens, Nuclear
Autoantigens
Bacterial Proteins
Cell Line
Cell Nucleus
Cricetinae
Energy Metabolism
Humans
Leukemia, Promyelocytic, Acute
Luminescent Proteins
Microscopy, Fluorescence
Models, Biological
Movement
Myosins
Neoplasm Proteins
Nuclear Proteins
Oncogene Proteins, Fusion
Recombinant Fusion Proteins
Time Factors
Transcription Factors
Tumor Suppressor Proteins |
| Subjects: |
diseases & disorders > cancer
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression
organs, tissues, organelles, cell types and functions > tissues types and functions > transport > protein transport
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Spector lab
School of Biological Sciences > Publications |
| Depositing User: |
Matt Covey
|
| Date: |
2002 |
| Date Deposited: |
10 Dec 2012 19:57 |
| Last Modified: |
29 Jan 2015 16:36 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/26352 |
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