Heard, E., Rougeulle, C., Arnaud, D., Avner, P., Allis, C. D., Spector, D. L. (2001) Methylation of histone H3 at Lys-9 is an early mark on the X chromosome during X inactivation. Cell, 107 (6). pp. 727-738. ISSN 0092-8674
Abstract
Coating of the X chromosome by Xist RNA is an essential trigger for X inactivation. However, little is known about the early chromatin remodeling events that transform this signal into transcriptional silencing. Here we report that methylation of histone H3 lysine 9 on the inactive X chromosome occurs immediately after Xist RNA coating and before transcriptional inactivation of X-linked genes. X-chromosomal H3 Lys-9 methylation occurs during the same window of time as H3 Lys-9 hypoacetylation and H3 Lys-4 hypomethylation. Histone H3 modifications thus represent the earliest known chromatin changes during X inactivation. We also identify a unique "hotspot" of H3 Lys-9 methylation 5' to Xist, and we propose that this acts as a nucleation center for Xist RNA-dependent spread of inactivation along the X chromosome via H3 Lys-9 methylation.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | A Kinase Anchor Proteins Acetylation Adaptor Proteins, Signal Transducing Animals Cell Cycle Proteins Cell Differentiation Cells, Cultured Chromatin Chromosomal Proteins, Non-Histone Cyclin-Dependent Kinase Inhibitor p27 DNA-Binding Proteins Dosage Compensation, Genetic Enzyme Inhibitors Female Fibroblasts Gene Silencing Histones In Situ Hybridization, Fluorescence Male Methyl-CpG-Binding Protein 2 Methylation Mice Models, Biological Oncogene Proteins Proto-Oncogene Proteins RNA RNA, Untranslated Repressor Proteins Stem Cells Transcription Factors Tumor Suppressor Proteins X Chromosome |
| Subjects: | bioinformatics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein methylation > histone methylation |
| CSHL Authors: | |
| Communities: | CSHL labs > Spector lab |
| Depositing User: | Matt Covey |
| Date: | 2001 |
| Date Deposited: | 10 Dec 2012 19:57 |
| Last Modified: | 29 Jan 2015 16:46 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/26350 |
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