Misteli, T., Spector, D. L. (1999) RNA polymerase II targets pre-mRNA splicing factors to transcription sites in vivo. Molecular Cell, 3. pp. 697-705. ISSN 1097-2765
Abstract
Biochemical evidence indicates that pre-mRNA splicing factors physically interact with the C-terminal domain of the largest subunit of RNA polymerase II. We have investigated the in vivo function of this interaction. In mammalian cells, truncation of the CTD of RNA pol II LS prevents the targeting of the splicing machinery to a transcription site. In the absence of the CTD, pre-mRNA splicing is severely reduced. The presence of unspliced RNA alone is not sufficient for the accumulation of splicing factors at the transcription site, nor for its efficient splicing. Our results demonstrate a critical role for the CTD of RNA pol II LS in the intranuclear targeting of splicing factors to transcription sites in vivo.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amanitins Animals Binding Sites Cell Nucleus Dichlororibofuranosylbenzimidazole Exons HeLa Cells Humans Nuclear Proteins Phosphoproteins Phosphorylation Protein Binding RNA RNA Polymerase II RNA Splicing RNA-Binding Proteins Rats Ribonucleoproteins Ribonucleoproteins, Small Nuclear Sequence Deletion Spliceosomes Transcription, Genetic |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA |
| CSHL Authors: | |
| Communities: | CSHL labs > Spector lab |
| Depositing User: | Matt Covey |
| Date: | 1999 |
| Date Deposited: | 10 Dec 2012 19:57 |
| Last Modified: | 29 Jan 2015 17:16 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/26345 |
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