Dynamics of transcription and pre-mRNA splicing within the mammalian cell nucleus

Spector, D. L., O'Keefe, R. T., Jimenez-Garcia, L. F. (1993) Dynamics of transcription and pre-mRNA splicing within the mammalian cell nucleus. Cold Spring Harbor Symposia on Quantitative Biology, 58. pp. 799-805. ISSN 00917451 (ISSN)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/7956094

DOI: 10.1101/SQB.1993.058.01.087

Abstract

Splicing is an essential step in the processing of intron-containing pre-mRNAs prior to their transport out of the nucleus (for review, see Green 1991). During the last few years, a significant number of studies have addressed the relationship of pre-mRNA splicing to nuclear structure (for review, see Spector 1993). SnRNPs as well as several non-snRNP splicing factors have been shown by immunofluorescence microscopy to be localized in a speckled pattern that is distributed in various regions throughout the nucleoplasm. Poly(A)+ RNA has been shown to colocalize with these nuclear speckles (Carter et al. 1991, 1993). Several specific RNAs have been localized in association with, the periphery of one or more speckles in a particular cell nucleus (Huang and Spector 1991; Xing et al. 1993). At the electron microscopic level, the speckled pattern corresponds to interchromatin granule clusters and perichromatin fibrils (Perraud et al. 1979; Fakan et al. 1984; Puvion et...

Item Type:	Paper
Uncontrolled Keywords:	Animals Base Sequence CHO Cells Cell Line Cell Nucleus Cricetinae DNA Probes Fluorescent Antibody Technique HeLa Cells Humans Microscopy, Immunoelectron Models, Biological Molecular Sequence Data RNA Precursors RNA Splicing Transcription, Genetic
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics organs, tissues, organelles, cell types and functions > organelles, types and functions > nucleus bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA
CSHL Authors:	Spector, David L.
Communities:	CSHL labs > Spector lab
Depositing User:	Matt Covey
Date:	1993
Date Deposited:	10 Dec 2012 19:44
Last Modified:	29 Jan 2015 19:33
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/26327

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