Walhout, A. J., Sordella, R., Lu, X., Hartley, J. L., Temple, G. F., Brasch, M. A., Thierry-Mieg, N., Vidal, M. (January 2000) Protein interaction mapping in C. elegans using proteins involved in vulval development. Science, 287 (5450). pp. 116-22. ISSN 0036-8075 (Print)
Abstract
Protein interaction mapping using large-scale two-hybrid analysis has been proposed as a way to functionally annotate large numbers of uncharacterized proteins predicted by complete genome sequences. This approach was examined in Caenorhabditis elegans, starting with 27 proteins involved in vulval development. The resulting map reveals both known and new potential interactions and provides a functional annotation for approximately 100 uncharacterized gene products. A protein interaction mapping project is now feasible for C. elegans on a genome-wide scale and should contribute to the understanding of molecular mechanisms in this organism and in human diseases.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals Caenorhabditis elegans genetics growth & development metabolism Caenorhabditis elegans Proteins Cloning Molecular Databases Factual Female Genes Helminth Genetic Vectors Genome Helminth Proteins genetics metabolism Mutation Open Reading Frames Phenotype Repressor Proteins genetics metabolism Retinoblastoma Protein genetics metabolism Two-Hybrid System Techniques Vulva growth & development |
| Subjects: | organism description > animal > C elegans bioinformatics > genomics and proteomics > annotation > map annotation bioinformatics > genomics and proteomics > design > protein network design bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein characterization bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression |
| CSHL Authors: | |
| Communities: | CSHL labs > Sordella lab |
| Depositing User: | CSHL Librarian |
| Date: | 7 January 2000 |
| Date Deposited: | 07 May 2012 21:07 |
| Last Modified: | 13 Mar 2013 16:21 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/26243 |
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