Detection of genetic loss in tumors by representational difference analysis

Lisitsyn, N. A., Leach, F. S., Vogelstein, B., Wigler, M. H. (1994) Detection of genetic loss in tumors by representational difference analysis. Cold Spring Harbor Symposia on Quantitative Biology, 59. pp. 585-7. ISSN 00917451 (ISSN)

DOI: 10.1101/SQB.1994.059.01.066


A variety of genetic lesions are found in tumors, including DNA losses, point mutations, gene amplifications, and rearrangements (Lasko et al. 1991; Salomon et al. 1991). Frequent losses of both alleles at a given locus or losses of one allele with functional inactivation of the other have been detected in many tumor types. These genetic lesions, manifesting themselves as loss of heterozygosity (LOH) and hemizygous and homozygous deletions, have been found to be the hallmarks of the presence of tumor suppressor genes. Many approaches have been taken in the past to identify these genes, but recently we have developed a new method that is both general and efficient (Lisitsyn et al. 1993, 1995). The method, called representational difference analysis, or RDA, is designed for analyzing the differences between complex but highly related genomes and combines three elements: representation, subtractive enrichment, and kinetic enrichment.

Item Type: Paper
Uncontrolled Keywords: Barrett Esophagus genetics Carcinoma Renal Cell genetics Chromosome Aberrations Chromosome Deletion Chromosomes Human Pair 3 Cloning Molecular Colorectal Neoplasms genetics Female Gene Deletion Homozygote Humans Kidney Neoplasms genetics Male Neoplasms genetics Tumor Cells Cultured
Subjects: diseases & disorders > cancer
Investigative techniques and equipment
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: 1994
Date Deposited: 12 Apr 2012 14:21
Last Modified: 24 Feb 2017 21:35
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