Maroney, P. A., Hannon, G. J., Denker, J. A., Nilsen, T. W. (1990) The nematode spliced leader RNA participates in trans-splicing as an Sm snRNP. Embo Journal, 9 (11). pp. 3667-3673. ISSN 0261-4189
Abstract
The trans-spliced leader RNA (SL RNA) of nematodes resembles U snRNAs both in cap structure and in the presence of a consensus Sm binding site. We show here that synthetic SL RNA, synthesized by in vitro transcription, is efficiently used as a spliced leader donor in trans-splicing reactions catalyzed by a cell free extract prepared from developing embryos of the parasitic nematode, Ascaris lumbricoides. Efficient utilization of synthetic SL RNA requires a functional Sm binding site. Mutations within the Sm binding sequence that prevent immunoprecipitation by Sm antisera and prevent cap trimethylation abolish trans-splicing. The effect on trans-splicing is not due to undermethylation of the cap structure.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Ascaris lumbricoides Sm antigens snRNP spliced leader RNA trans splicing ribonucleoprotein article ascaris nematode nonhuman priority journal rna splicing Animal Autoantigens Base Sequence Binding Sites DNA Mutational Analysis In Vitro Molecular Sequence Data Nucleic Acid Precursors Oligonucleotides Ribonucleoproteins Ribonucleoproteins Small Nuclear RNA Caps RNA Messenger Nematoda |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA splicing bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > shRNA bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > siRNA |
| CSHL Authors: | |
| Communities: | CSHL labs > Hannon lab |
| Depositing User: | Editor Margaret Fantz |
| Date: | 1990 |
| Date Deposited: | 17 Apr 2012 17:31 |
| Last Modified: | 02 Dec 2016 19:31 |
| PMCID: | PMC552121 |
| URI: | https://repository.cshl.edu/id/eprint/26122 |
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