New polymorphic markers in the vicinity of the pearl locus on mouse chromosome 13

Xu, H. P., Yanak, B. L., Wigler, M. H., Gorin, M. B. (January 1996) New polymorphic markers in the vicinity of the pearl locus on mouse chromosome 13. Mamm Genome, 7 (1). pp. 16-9. ISSN 0938-8990 (Print)

DOI: 10.1007/s003359900005


We have used a Mus domesticus/-Mus spretus congenic animal that was selected for retention of Mus spretus DNA around the pearl locus to create a highly polymorphic region suitable for screening new markers. Representation difference analysis (RDA) was performed with either DNA from the congenic animal or C57BL/6J as the driver for subtraction. Four clones were identified, characterized, and converted to PCR-based polymorphic markers. Three of the four markers equally subdivide a 10-cM interval containing the pearl locus, with the fourth located centromeric to it. These markers have been placed on the mouse genetic map by use of an interspecific backcross panel between Mus domesticus (C57BL/6J) and Mus spretus generated by The Jackson Laboratory.

Item Type: Paper
Uncontrolled Keywords: Animals Base Sequence Blotting Southern Chromosome Mapping Cloning Molecular Crosses Genetic DNA Primers chemistry Genetic Markers genetics Mice Mice Inbred Strains Molecular Sequence Data Polymerase Chain Reaction Polymorphism Genetic genetics
Subjects: Investigative techniques and equipment > cloning > PCR
Investigative techniques and equipment > assays > cloning > PCR
bioinformatics > genomics and proteomics > annotation > sequence annotation
bioinformatics > genomics and proteomics > Mapping and Rendering > Sequence Rendering
Investigative techniques and equipment > biomarker
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: January 1996
Date Deposited: 10 Apr 2012 14:25
Last Modified: 18 Nov 2016 17:37
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