Jackson, D. P. (August 2002) Double labeling of KNOTTED1 mRNA and protein reveals multiple potential sites of protein trafficking in the shoot apex. Plant Physiology, 129 (4). pp. 1423-1429. ISSN 0032-0889
Abstract
Recent reports indicate that several plant mRNAs and proteins are able to traffic intercellularly through plasmodesmata. Localization studies can reveal differences between mRNA and protein localization that would be indicative of such a process. However, subtle differences could be missed when comparing localization in adjacent sections, especially in developmental studies where adjacent sections through immature apical regions may be one or more cells removed from each other. Therefore, we have developed a novel method for double localization of KNOTTED1 mRNA and protein in sections through the maize (Zea mays) shoot apex. The advantage of double labeling is revealed in our demonstration of novel potential sites of cell-to-cell trafficking of KNOTTED1 protein in the shoot apical region. The technique should be applicable to any gene products where the appropriate probes are available and will, therefore, help to determine the extent of protein and/or mRNA trafficking in plants.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > knotted1 bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > knotted1 bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mRNA dynamics |
| CSHL Authors: | |
| Communities: | CSHL labs > Jackson lab |
| Depositing User: | CSHL Librarian |
| Date: | August 2002 |
| Date Deposited: | 03 Apr 2012 20:28 |
| Last Modified: | 11 Jan 2017 21:49 |
| PMCID: | PMC1540245 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25914 |
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