Control of Arabidopsis meristem development by thioredoxin-dependent regulation of intercellular transport

Benitez-Alfonso, Y., Cilia, M., Roman, A. S., Thomas, C., Maule, A., Hearn, S., Jackson, D. P. (March 2009) Control of Arabidopsis meristem development by thioredoxin-dependent regulation of intercellular transport. Proc Natl Acad Sci U S A, 106 (9). pp. 3615-3620. ISSN 0027-8424

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DOI: 10.1073/pnas.0808717106


Cell-to-cell transport in plants occurs through cytoplasmic channels called "plasmodesmata'' and is regulated by developmental and environmental factors. Callose deposition modulates plasmodesmal transport in vivo, but little is known about the mechanisms that regulate this process. Here we report a genetic approach to identify mutants affecting plasmodesmal transport. We isolated 5 mutants, named gfp arrested trafficking (gat), affected in GFP unloading from the phloem into the meristem. gat1 mutants were seedling lethal and carried lesions in an m-type thioredoxin that is expressed in non-green plastids of meristems and organ primordia. Callose and hydrogen peroxide accumulated in gat1 mutants, and WT plants subjected to oxidative conditions phenocopied the gat1 trafficking defects. Ectopic expression of GAT1 in mature leaves increased plasmodesmal permeability and led to a delay in senescence and flowering time. We propose a role for the GAT1 thioredoxin in the redox regulation of callose deposition and symplastic permeability that is essential for meristem maintenance in Arabidopsis.

Item Type: Paper
Uncontrolled Keywords: callose cell to cell trafficking plasmodesmata redox regulation thioredoxin m type m-type GREEN FLUORESCENT PROTEIN green flourescent protein CELL-TO-CELL cell-to-cell OXIDATIVE STRESS oxidative stress TRANSCRIPTION FACTORS transcription factors CALLOSE DEPOSITION callose deposition TOBACCO-LEAVES tobacco leaves PLASMODESMATA plasmodesmata PLANT plant trafficking TRAFFICKING MOVEMENT
Subjects: organism description > plant > Arabidopsis
organs, tissues, organelles, cell types and functions > tissues types and functions > meristem
organism description > plant behavior > plasmodesmata
organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > plasmodesmata
CSHL Authors:
Communities: CSHL labs > Jackson lab
School of Biological Sciences > Publications
CSHL Cancer Center Shared Resources > Microscopy Service
Depositing User: CSHL Librarian
Date: 3 March 2009
Date Deposited: 04 Apr 2012 14:37
Last Modified: 08 Nov 2017 20:52
PMCID: PMC2651306
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