Taniguchi, H., He, M., Wu, P., Kim, S., Paik, R., Sugino, K., Kvitsani, D., Fu, Y., Lu, J., Lin, Y., Miyoshi, G., Shima, Y., Fishell, G., Nelson, S. B., Huang, Z. J. (September 2011) A Resource of Cre Driver Lines for Genetic Targeting of GABAergic Neurons in Cerebral Cortex. Neuron, 71 (6). pp. 995-1013. ISSN 0896-6273
Abstract
Summary A key obstacle to understanding neural circuits in the cerebral cortex is that of unraveling the diversity of GABAergic interneurons. This diversity poses general questions for neural circuit analysis: how are these interneuron cell types generated and assembled into stereotyped local circuits and how do they differentially contribute to circuit operations that underlie cortical functions ranging from perception to cognition? Using genetic engineering in mice, we have generated and characterized approximately 20 Cre and inducible CreER knockin driver lines that reliably target major classes and lineages of GABAergic neurons. More select populations are captured by intersection of Cre and Flp drivers. Genetic targeting allows reliable identification, monitoring, and manipulation of cortical GABAergic neurons, thereby enabling a systematic and comprehensive analysis from cell fate specification, migration, and connectivity, to their functions in network dynamics and behavior. As such, this approach will accelerate the study of GABAergic circuits throughout the mammalian brain.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | INTERNEURON DIVERSITY SERIES interneuron diversity series CENTRAL-NERVOUS-SYSTEM central nervous system CORTICAL INTERNEURONS cortical interneurons HIPPOCAMPAL INTERNEURONS hippocampal interneurons IN-VIVO in-vivo SOMATOSENSORY CORTEX somatosensory cortex RAT rat EXPRESSION expression CELLS cells TRANSCRIPTION transcription |
| Subjects: | bioinformatics > genomics and proteomics > small molecules > GABAergic organs, tissues, organelles, cell types and functions > tissues types and functions > cerebral cortex neurobiology organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons |
| CSHL Authors: | |
| Communities: | CSHL labs > Huang lab CSHL Post Doctoral Fellows |
| Depositing User: | CSHL Librarian |
| Date: | 22 September 2011 |
| Date Deposited: | 10 Apr 2012 16:38 |
| Last Modified: | 07 Apr 2015 16:11 |
| PMCID: | PMC3779648 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25814 |
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