Huang, Z. J., Yu, W., Lovett, C., Tonegawa, S.
(2002)
Cre/loxP recombination-activated neuronal markers in mouse neocortex and hippocampus.
Genesis, 32 (3).
pp. 209-217.
ISSN 1526954X (ISSN)
Abstract
A new strategy for visualizing neuronal cell morphology of mouse brain based on Cre/loxP recombination-activated gene expression is described. A "reporter" transgenic line was generated which expressed a fusion gene encoding a dendrite-targeted green fluorescent protein (MAP2-GFP) upon deletion of a transcription/translation STOP (transcription and translation stop signal) cassette. Cre transgenic "deleter" lines were established that activated reporter gene expression at various frequencies in pyramidal neurons in the forebrain. A deleter line was identified which activated a MAP2-GFP reporter gene at very low frequency (less than 0.1% of pyramidal neurons) and allowed the visualization of dendritic structures of individual neocortical and hippocampal pyramidal neurons. In addition, vertical "columns" of pyramidal neurons in the neocortex were labeled in these mice. In a second deleter line, a MAP2-GFP reporter gene was selectively activated in pyramidal neurons of the CA-1 subregion of the hippocampus in young mice. With its combinatorial property, this binary recombination-activated neuronal marker system should facilitate the study of detailed morphology, connectivity, and plasticity of defined classes of live neurons in vitro and in vivo. © 2002 Wiley-Liss, Inc.
| Item Type: |
Paper
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| Uncontrolled Keywords: |
Cre/loxP
Green fluorescent protein GFP
Neocortex
Recombination
cre recombinase
gene product
green fluorescent protein
unclassified drug
animal tissue
article
cell structure
controlled study
dendrite
forebrain
fusion gene
gene activation
gene cassette
gene expression
gene frequency
gene targeting
genetic recombination
genetic transcription
hippocampus
marker gene
mouse
nerve cell
nonhuman
plasticity
priority journal
pyramidal nerve cell
reporter gene
RNA translation
signal transduction
Animals
Biological Markers
Gene Expression Regulation
Gene Transfer Techniques
Integrases
Mice
Mice, Transgenic
Microscopy, Confocal
Neuronal Plasticity
Neurons
Recombination, Genetic
Staining and Labeling
Viral Proteins |
| Subjects: |
Investigative techniques and equipment
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > green fluorescent protein
organism description > animal > mammal > rodent > mouse
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Huang lab |
| Depositing User: |
CSHL Librarian
|
| Date: |
2002 |
| Date Deposited: |
10 Apr 2012 15:45 |
| Last Modified: |
10 May 2013 19:01 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/25807 |
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