Bernard, D., Prasanth, K. V. , Tripathi, V., Colasse, S., Nakamura, T., Xuan, Z., Zhang, M. Q. , Sedel, F., Jourdren, L., Coulpier, F., Triller, A., Spector, D. L., Bessis, A. (2010) A long nuclear-retained non-coding RNA regulates synaptogenesis by modulating gene expression. EMBO Journal, 29 (18). pp. 3082-3093.
Abstract
A growing number of long nuclear-retained non-coding RNAs (ncRNAs) have recently been described. However, few functions have been elucidated for these ncRNAs. Here, we have characterized the function of one such ncRNA, identified as metastasis-associated lung adenocarcinoma transcript 1 (Malat1). Malat1 RNA is expressed in numerous tissues and is highly abundant in neurons. It is enriched in nuclear speckles only when RNA polymerase II-dependent transcription is active. Knock-down studies revealed that Malat1 modulates the recruitment of SR family pre-mRNA-splicing factors to the transcription site of a transgene array. DNA microarray analysis in Malat1-depleted neuroblastoma cells indicates that Malat1 controls the expression of genes involved not only in nuclear processes, but also in synapse function. In cultured hippocampal neurons, knock-down of Malat1 decreases synaptic density, whereas its over-expression results in a cell-autonomous increase in synaptic density. Our results suggest that Malat1 regulates synapse formation by modulating the expression of genes involved in synapse formation and/or maintenance.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > ncRNA |
| CSHL Authors: | |
| Communities: | CSHL labs > Spector lab CSHL labs > Zhang lab |
| Depositing User: | Brian Soldo |
| Date: | 2010 |
| Date Deposited: | 04 Apr 2012 19:11 |
| Last Modified: | 28 Jan 2015 15:59 |
| PMCID: | PMC2944070 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25644 |
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