Janas, M. L., Hodson, D., Stamataki, Z., Hill, S., Welch, K., Gambardella, L., Trotman, L. C., Pandolfi, P. P., Vigorito, E., Turner, M. (January 2008) The Effect of Deleting p110{delta} on the Phenotype and Function of PTEN-Deficient B Cells. Journal of Immunology, 180 (2). pp. 739-46. ISSN 0022-1767
Abstract
Control of the intracellular levels of phosphatidylinositol-(3, 4, 5)-trisphosphate by PI3K and phosphatase and tensin homolog (PTEN) is essential for B cell development and differentiation. Deletion of the PI3K catalytic subunit p110delta leads to a severe reduction in B1 and marginal zone (MZ) B cells, whereas deletion of PTEN results in their expansion. We have examined the relationship between these two molecules by generating mice with a B cell-specific deletion of PTEN (PTEN(B)) and a concurrent germline deletion of p110delta. The expanded B1 cell population of PTEN(B) mice was reduced to normal levels in PTEN(B)/p110delta mutant mice, indicating a critical role for the p110delta isoform in the expansion of B1 cells. However, numbers of MZ B cells in the PTEN(B)/p110delta mutants was intermediate between wild-type and PTEN(B)-deficient mice, suggesting an additional role for other PI3K catalytic isoforms in MZ differentiation. Furthermore, the defective class switch recombination in PTEN(B) B cells was only partially reversed in PTEN(B)/p110delta double mutant B cells. These results demonstrate an epistatic relationship between p110delta and PTEN. In addition, they also suggest that additional PI3K catalytic subunits contribute to B cell development and function.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > PTEN bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression |
| CSHL Authors: | |
| Communities: | CSHL labs > Trotman lab |
| Depositing User: | Brian Soldo |
| Date: | 15 January 2008 |
| Date Deposited: | 23 Mar 2012 16:07 |
| Last Modified: | 08 May 2013 16:33 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25520 |
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