Vakoc, C. R., Letting, D. L., Gheldof, N., Sawado, T., Bender, M. A., Groudine, M., Weiss, M. J., Dekker, J., Blobel, G. A.
(2005)
Proximity among distant regulatory elements at the β-globin locus requires GATA-1 and FOG-1.
Molecular Cell, 17 (3).
pp. 453-462.
ISSN 10972765 (ISSN)
Abstract
Recent evidence suggests that long-range enhancers and gene promoters are in close proximity, which might reflect the formation of chromatin loops. Here, we examined the mechanism for DNA looping at the β-globin locus. By using chromosome conformation capture (3C), we show that the hematopoietic transcription factor GATA-1 and its cofactor FOG-1 are required for the physical interaction between the β-globin locus control region (LCR) and the β-major globin promoter. Kinetic studies reveal that GATA-1-induced loop formation correlates with the onset of β-globin transcription and occurs independently of new protein synthesis. GATA-1 occupies the β-major globin promoter normally in fetal liver erythroblasts from mice lacking the LCR, suggesting that GATA-1 binding to the promoter and LCR are independent events that occur prior to loop formation. Together, these data demonstrate that GATA-1 and FOG-1 are essential anchors for a tissue-specific chromatin loop, providing general insights into long-range enhancer function.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
beta globin
transcription factor
transcription factor fog 1
transcription factor GATA 1
unclassified drug
article
erythroblast
fetus liver
gene interaction
gene locus
genetic transcription
promoter region
protein function
protein synthesis
regulator gene
Animals
Base Sequence
Binding Sites
Carrier Proteins
Cell Line
DNA
DNA-Binding Proteins
Enhancer Elements (Genetics)
Erythroid-Specific DNA-Binding Factors
GATA1 Transcription Factor
Genes, Regulator
Globins
Humans
Locus Control Region
Mice
Mice, Mutant Strains
Nuclear Proteins
Nucleic Acid Conformation
Promoter Regions (Genetics)
Protein Binding
RNA Polymerase II
Transcription Factors |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Vakoc lab |
| Depositing User: |
CSHL Librarian
|
| Date: |
2005 |
| Date Deposited: |
19 Mar 2012 19:07 |
| Last Modified: |
13 Mar 2013 20:36 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/25402 |
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