Hong, W., Nakazawa, M., Chen, Y. Y., Kori, R., Vakoc, C. R., Rakowski, C., Blobel, G. A.
(2005)
FOG-1 recruits the NuRD repressor complex to mediate transcriptional repression by GATA-1.
Embo Journal, 24 (13).
pp. 2367-2378.
ISSN 0261-4189
Abstract
Transcription factor GATA-1 and its cofactor FOG-1 coordinate erythroid cell maturation by activating erythroid-specific genes and repressing genes associated with the undifferentiated state. Here we show that FOG-1 binds to the NuRD corepressor complex in vitro and in vivo. The interaction is mediated by a small conserved domain at the extreme N-terminus of FOG-1 that is necessary and sufficient for NuRD binding. This domain defines a novel repression module found in diverse transcriptional repressors. NuRD is present at GATA-1/FOG-1-repressed genes in erythroid cells in vivo. Point mutations near the N-terminus of FOG-1 that abrogate NuRD binding block gene repression by FOG-1. Finally, the ability of GATA-1 to repress transcription was impaired in erythroid cells expressing mutant forms of FOG-1 that are defective for NuRD binding. Together, these studies show that FOG-1 and likely other FOG-like proteins are corepressors that link GATA factors to histone deacetylation and nucleosome remodeling. © 2005 European Molecular Biology Organization | All Rights Reserved.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
Chromatin
FOG-1
GATA-1
Gene repression
NuRD
histone deacetylase
protein fog 1
transcription factor GATA 1
unclassified drug
zinc finger protein
amino terminal sequence
animal cell
article
cell maturation
controlled study
deacetylation
erythroid cell
human
human cell
nonhuman
nucleosome
NuRD repressor complex
point mutation
priority journal
protein binding
protein domain
protein expression
protein protein interaction
repressor gene
transcription regulation
transcription repression
Amino Acid Motifs
Amino Acid Sequence
Animals
Carrier Proteins
Cells, Cultured
Cercopithecus aethiops
DNA-Binding Proteins
Erythroid Specific DNA Binding Factors
GATA1 Transcription Factor
Gene Silencing
Histone Deacetylases
Mice
Molecular Sequence Data
N-Glycosyl Hydrolases
Nuclear Proteins
Protein Structure, Tertiary
Proto-Oncogene Proteins c-kit
Repressor Proteins
Sequence Homology, Amino Acid
Transcription Factors
Transcription, Genetic |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein receptor
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Vakoc lab |
| Depositing User: |
CSHL Librarian
|
| Date: |
2005 |
| Date Deposited: |
19 Mar 2012 18:28 |
| Last Modified: |
13 Mar 2013 20:32 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/25395 |
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