Decker, D. J., Linton, P. J., Zaharevitz, S., Biery, M., Gingeras, T. R., Klinman, N. R. (1995) Defining subsets of naive and memory B cells based on the ability of their progeny to somatically mutate in vitro. Immunity, 2 (2). pp. 195-203. ISSN 10747613 (ISSN)
Abstract
The increased affinity of memory antibody responses is due largely to the generation and selection of memory B cells that accumulate somatic mutations after initial antigenic stimulation. Further affinity maturation and mutation also accompany subsequent immunizations. Previous studies have suggested that, like primary antibody-forming cell (AFC) clones, secondary AFC do not accumulate further mutations and, therefore, the origins of progressive affinity maturation remain controversial. Here, we report the generation of somatically mutated memory B cell clones in vitro. Our findings confirm the existence of a naive B cell subset whose progeny, rather than generating AFC, somatically mutate and respond to subsequent antigenic stimulation. Interestingly, upon stimulation, a subset of memory B cells also generates antigen-responsive cells that accumulate further somatic mutations.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | animal cell animal experiment antibody producing cell antibody response article b lymphocyte cell clone dna sequence immunization memory cell mouse nonhuman phenotype priority journal somatic mutation Amino Acid Sequence Animal Antibody Formation B-Lymphocyte Subsets Base Sequence Cells Cultured Immunologic Memory Lymphocyte Subsets Mice Molecular Sequence Data Mutation RNA Stem Cells |
| Subjects: | organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > immunity |
| CSHL Authors: | |
| Communities: | CSHL labs > Gingeras lab |
| Depositing User: | CSHL Librarian |
| Date: | 1995 |
| Date Deposited: | 13 Mar 2012 15:46 |
| Last Modified: | 28 Mar 2014 20:06 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25259 |
Actions (login required)
 |
Administrator's edit/view item |