Ellis, S. B., Brust, P. F., Koutz, P. J., Waters, A. F., Harpold, M. M., Gingeras, T. R. (May 1985) Isolation of alcohol oxidase and two other methanol regulatable genes from the yeast Pichia pastoris. Mol Cell Biol, 5 (5). pp. 1111-21. ISSN 0270-7306 (Print)0270-7306 (Linking)
Abstract
The oxidation of methanol follows a well-defined pathway and is similar for several methylotrophic yeasts. The use of methanol as the sole carbon source for the growth of Pichia pastoris stimulates the expression of a family of genes. Three methanol-responsive genes have been isolated; cDNA copies have been made from mRNAs of these genes, and the protein products from in vitro translations have been examined. The identification of alcohol oxidase as one of the cloned, methanol-regulated genes has been made by enzymatic, immunological, and sequence analyses. Methanol-regulated expression of each of these three isolated genes can be demonstrated to occur at the level of transcription. Finally, DNA subfragments of two of the methanol-responsive genomic clones from P. pastoris have been isolated and tentatively identified as containing the control regions involved in methanol regulation.
| Item Type: | Paper |
|---|---|
| Additional Information: | |
| Uncontrolled Keywords: | Alcohol Oxidoreductases genetics Ascomycota genetics Base Sequence DNA genetics DNA, Fungal isolation & purification Gene Expression Regulation drug effects Genes Fungal Genes Regulator Methanol metabolism pharmacology Pichia enzymology genetics Promoter Regions Genetic Transcription Genetic drug effects |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > translation organism description > yeast |
| CSHL Authors: | |
| Communities: | CSHL labs > Gingeras lab |
| Depositing User: | CSHL Librarian |
| Date: | May 1985 |
| Date Deposited: | 14 Mar 2012 15:10 |
| Last Modified: | 31 Mar 2014 13:54 |
| PMCID: | PMC366829 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25235 |
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