Sanders, C. M., Stenlund, A. (December 1998) Recruitment and loading of the E1 initiator protein: an ATP-dependent process catalysed by a transcription factor. Embo J, 17 (23). pp. 7044-55. ISSN 0261-4189 (Print)
Abstract
Initiation of DNA replication critically depends on ori recognition as well as on catalytic activities of the initiator complex. For replication of papillomaviruses the catalytic activities for initiation are provided by the E1 protein. Here, we show that the transcription factor E2 acts to assemble E1 into a complex active for ori distortion in two steps. First, cooperative DNA binding of E1 and E2 generates a sequence-specific ori recognition complex. In the second ATP-dependent step, E2 is displaced and additional E1 molecules are incorporated. The net result is a final complex with low sequence specificity deposited onto a specific sequence in the DNA. This may be a general strategy to accomplish specific positioning of protein complexes with low sequence specificity.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Adenosine Triphosphate metabolism Animals Catalysis Cattle DNA Footprinting DNA-Binding Proteins metabolism Deoxyribonuclease I Magnesium Replication Origin Research Support Non US Govt Research Support US Govt PHS Transcription Factors metabolism Transcription Genetic Viral Proteins metabolism |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor organism description > virus |
| CSHL Authors: | |
| Communities: | CSHL labs > Stenlund lab |
| Depositing User: | Brian Soldo |
| Date: | 1 December 1998 |
| Date Deposited: | 15 Mar 2012 15:50 |
| Last Modified: | 14 Feb 2017 20:22 |
| PMCID: | PMC1171052 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25192 |
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