Stenlund, A. (February 2003) E1 initiator DNA binding specificity is unmasked by selective inhibition of non-specific DNA binding. Embo Journal, 22 (4). pp. 954-963. ISSN 0261-4189
Abstract
Initiator proteins are critical components of the DNA replication machinery and mark the site of initiation. This activity probably requires highly selective DNA binding; however, many initiators display modest specificity in vitro. We demonstrate that low specificity of the papillomavirus E1 initiator results from the presence of a non-specific DNA-binding activity, involved in melting, which masks the specificity intrinsic to the E1 DNA-binding domain. The viral factor E2 restores specificity through a physical interaction with E1 that suppresses non-specific binding. We propose that this arrangement, where one DNA-binding activity tethers the initiator to ori while another alters DNA structure, is a characteristic of other viral and cellular initiator proteins. This arrangement would provide an explanation for the low selectivity observed for DNA binding by initiator proteins.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | DNA binding DNA replication initiator specificity factor ORIGIN RECOGNITION COMPLEX origin recognition complex LARGE T-ANTIGEN large T-antigen BOVINE PAPILLOMAVIRUS bovine papillomavirus E2 PROTEINS TRANSCRIPTION FACTOR transcription factor STRUCTURAL CHANGES REPLICATION replication DOMAIN domain ATP HELICASE helicase |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein organism description > virus > papillomavirus |
| CSHL Authors: | |
| Communities: | CSHL labs > Stenlund lab |
| Depositing User: | Brian Soldo |
| Date: | February 2003 |
| Date Deposited: | 15 Mar 2012 14:05 |
| Last Modified: | 14 Feb 2017 20:06 |
| PMCID: | PMC145451 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25181 |
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