Schuck, S., Stenlund, A. (July 2005) Role of papillomavirus E1 initiator dimerization in DNA replication. J Virol, 79 (13). pp. 8661-4. ISSN 0022-538X (Print)
Abstract
Viral initiator proteins are polypeptides that form oligomeric complexes on the origin of DNA replication (ori). These complexes carry out a multitude of functions related to initiation of DNA replication, and although many of these functions have been characterized biochemically, little is understood about how the complexes are assembled. Here we demonstrate that loss of one particular interaction, the dimerization between E1 DNA binding domains, has a severe effect on DNA replication in vivo but has surprisingly modest effects on most individual biochemical activities in vitro. We conclude that the dimer interaction is primarily required for initial recognition of ori.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amino Acid Substitution Animals Bovine papillomavirus 1 genetics Cattle DNA Replication DNA-Binding Proteins genetics metabolism Dimerization Escherichia coli genetics Kinetics Papillomaviridae genetics Recombinant Proteins metabolism Viral Proteins genetics metabolism |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein |
| CSHL Authors: | |
| Communities: | CSHL labs > Stenlund lab |
| Depositing User: | Brian Soldo |
| Date: | July 2005 |
| Date Deposited: | 15 Mar 2012 14:11 |
| Last Modified: | 01 Mar 2013 20:34 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25179 |
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