Zou, L., Stillman, B. (April 1998) Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin. Science, 280 (5363). pp. 593-6. ISSN 0036-8075 (Print)
Abstract
Cdc45p, a protein essential for initiation of DNA replication, associates with chromatin after "start" in late G1 and during the S phase of the cell cycle. Binding of Cdc45p to chromatin depends on Clb-Cdc28 kinase activity as well as functional Cdc6p and Mcm2p, which suggests that Cdc45p associates with the prereplication complex after activation of S-phase cyclin-dependent kinases (CDKs). As indicated by the timing and the CDK dependence, binding of Cdc45p to chromatin is crucial for commitment to initiation of DNA replication. During S phase, Cdc45p physically interacts with minichromosome maintenance (MCM) proteins on chromatin; however, dissociation of Cdc45p from chromatin is slower than that of MCMs, which indicates that the proteins are released by different mechanisms.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | CDC28 Protein Kinase S cerevisiae metabolism Carrier Proteins metabolism Cell Cycle Proteins metabolism Chromatin metabolism Cyclin-Dependent Kinases metabolism Cyclins metabolism DNA Replication DNA, Fungal biosynthesis DNA-Binding Proteins metabolism Enzyme Activation Fungal Proteins metabolism G1 Phase Nuclear Proteins metabolism Origin Recognition Complex S Phase Saccharomyces cerevisiae cytology metabolism Saccharomyces cerevisiae Proteins |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics |
| CSHL Authors: | |
| Communities: | CSHL labs > Stillman lab |
| Highlight: | Stillman, Bruce W. |
| Depositing User: | CSHL Librarian |
| Date: | 24 April 1998 |
| Date Deposited: | 17 Feb 2012 15:20 |
| Last Modified: | 20 Jun 2017 19:28 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25151 |
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