Williams, R. S., Shohet, R. V., Stillman, B. (January 1997) A human protein related to yeast Cdc6p. Proceedings of the National Academy of Sciences of the United States of America, 94 (1). pp. 142-7. ISSN 0027-8424
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Abstract
The unstable proteins Cdc6p and cdc18+ are essential and rate limiting for the initiation of DNA replication in Saccharomyces cerevisiae and Schizosaccharomyces pombe, respectively, and also participate in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. We have identified Xenopus and human proteins closely related to Cdc6p/cdc18. The human protein, p62(cdc6), is encoded on chromosome 17q21.3 and includes putative cyclin-dependent kinase phosphorylation sites, destruction boxes, a nucleotide binding/ATPase domain, and a potential leucine zipper. Expression of p62(cdc6) mRNA and protein is suppressed in human diploid fibroblasts made quiescent by serum starvation, and peaks as cells reenter the cell cycle and replicate DNA following serum stimulation. Conservation of structure among proteins involved in initiation suggests that fundamental features of replication complexes are maintained in all eukaryotes.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amino Acid Sequence Animals Base Sequence Cell Compartmentation Cell Cycle Proteins biosynthesis genetics Cell Nucleus chemistry Chromosome Mapping Chromosomes Human, Pair 17 Cloning Molecular Comparative Study Conserved Sequence DNA Replication DNA Complementary genetics Fungal Proteins genetics Gene Expression Humans Molecular Sequence Data Nuclear Proteins biosynthesis genetics RNA Messenger biosynthesis Schizosaccharomyces pombe Proteins Sequence Analysis DNA Sequence Homology Amino Acid Species Specificity Transcription Genetic Xenopus |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication |
| CSHL Authors: | |
| Communities: | CSHL labs > Stillman lab |
| Highlight: | Stillman, Bruce W. |
| Depositing User: | CSHL Librarian |
| Date: | 7 January 1997 |
| Date Deposited: | 17 Feb 2012 19:50 |
| Last Modified: | 11 Sep 2019 17:01 |
| PMCID: | PMC19260 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25143 |
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