Waga, S., Stillman, B. (May 1994) Anatomy of a DNA replication fork revealed by reconstitution of SV40 DNA replication in vitro. Nature, 369 (6477). pp. 207-12. ISSN 0028-0836 (Print)
Abstract
Complete enzymatic replication of DNA from the simian virus 40 origin has been reconstituted with T antigen and highly purified cellular proteins. DNA polymerase-alpha/primase functions primarily to synthesize RNA-DNA primers for initiation of DNA replication at the origin and for priming each Okazaki fragment. A polymerase switching mechanism requiring replication factor C and the proliferating cell nuclear antigen allows two molecules of DNA polymerase-delta to replicate both strands of the double helix conjointly.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals Cattle DNA Replication DNA Viral biosynthesis genetics DNA-Binding Proteins metabolism DNA-Directed DNA Polymerase metabolism Homeodomain Proteins Humans Nuclear Proteins metabolism Proliferating Cell Nuclear Antigen Proto-Oncogene Proteins c-bcl-2 Replication Factor A Replication Factor C Repressor Proteins Saccharomyces cerevisiae Proteins Simian virus 40 genetics Templates Genetic Viral Proteins metabolism |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > RNA polymerase |
| CSHL Authors: | |
| Communities: | CSHL labs > Stillman lab |
| Highlight: | Stillman, Bruce W. |
| Depositing User: | CSHL Librarian |
| Date: | 19 May 1994 |
| Date Deposited: | 17 Feb 2012 20:44 |
| Last Modified: | 20 Jun 2017 19:49 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25130 |
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