Van Dyck, E., Foury, F., Stillman, B., Brill, S. J. (September 1992) A Single-Stranded-DNA Binding-Protein Required for Mitochondrial-DNA Replication in Saccharomyces-Cerevisiae Is Homologous to Escherichia-Coli Ssb. Embo Journal, 11 (9). pp. 3421-3430. ISSN 0261-4189
Abstract
It has previously been shown that the mitochondrial DNA (mtDNA) of Saccharomyces cerevisiae becomes thermosensitive due to the inactivation of the mitochondrial DNA helicase gene, PIF1. A suppressor of this thermosensitive phenotype was isolated from a wild-type plasmid library by transforming a pif1 null strain to growth on glycerol at the non-permissive temperature. This suppressor is a nuclear gene encoding a 135 amino acid protein that is itself essential for mtDNA replication; cells lacking this gene are totally devoid of mtDNA. We therefore named this gene RIM1 for replication in mitochondria. The primary structure of he RIM1 protein is homologous to the single-stranded DNA binding protein (SSB) from Escherichia coli and to the mitochondrial SSB from Xenopus laevis. The mature RIM1 gene product has been purified from yeast extracts using a DNA unwinding assay dependent upon the DNA helicase activity of SV40 T-antigen. Direct amino acid sequencing of the protein reveals that RIM1 is a previously uncharacterized SSB. Antibodies against this purified protein localize RIM1 to mitochondria. The SSB encoded by RIM1 is therefore an essential component of the yeast mtDNA replication apparatus.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | DNA HELICASE DNA Helicase MITOCHONDRIAL DNA REPLICATION Mitochondrial DNA Replication SINGLE-STRANDED DNA single stranded DNA BINDING PROTEIN binding protein YEAST yeast ESCHERICHIA-COLI Escherichia Coli SACCHAROMYCES-CEREVISIAE Saccharomyces Cerevisiae YEAST MITOCHONDRIA yeast mitochondria SV40 ORIGIN origin FACTOR-A Factor-A GENE TRANSCRIPTION transcription SEQUENCE sequence nuclear NUCLEAR POLYMERASE polymerase |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication organs, tissues, organelles, cell types and functions > organelles, types and functions > mitochondria |
| CSHL Authors: | |
| Communities: | CSHL labs > Stillman lab |
| Highlight: | Stillman, Bruce W. |
| Depositing User: | CSHL Librarian |
| Date: | September 1992 |
| Date Deposited: | 28 Feb 2012 20:01 |
| Last Modified: | 20 Jun 2017 20:00 |
| PMCID: | PMC556877 |
| URI: | https://repository.cshl.edu/id/eprint/25123 |
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