Tsurimoto, T., Stillman, B. (January 1991) Replication factors required for SV40 DNA replication in vitro. II. Switching of DNA polymerase alpha and delta during initiation of leading and lagging strand synthesis. Journal of Biological Chemistry, 266 (3). pp. 1961-8. ISSN 0021-9258
Abstract
Replication factors A and C (RF-A and RF-C) and the proliferating cell nuclear antigen (PCNA) differentially augment the activities of DNA polymerases alpha and delta. The mechanism of stimulation by these replication factors was investigated using a limiting concentration of primed, single-stranded template DNA. RF-A stimulated polymerase alpha activity in a concentration-dependent manner, but also suppressed nonspecific initiation of DNA synthesis by both polymerases alpha and delta. The primer recognition complex, RF-C.PCNA.ATP, stimulated pol delta activity in cooperation with RF-A, but also functioned to prevent abnormal initiation of DNA synthesis by polymerase alpha. Reconstitution of DNA replication with purified factors and a plasmid containing the SV40 origin sequences directly demonstrated DNA polymerase alpha dependent synthesis of lagging strands and DNA polymerase delta/PCNA/RF-C dependent synthesis of leading strands. RF-A and the primer recognition complex both affected the relative levels of leading and lagging strands. These results, in addition to results in an accompanying paper (Tsurimoto, T., and Stillman, B. (1991) J. Biol. Chem. 266, 1950-1960), suggest that an exchange of DNA polymerase complexes occurs during initiation of bidirectional DNA replication at the SV40 origin.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Adenosine Triphosphate metabolism DNA Polymerase II metabolism DNA Polymerase III DNA Primase DNA Replication DNA, Viral biosynthesis DNA-Binding Proteins physiology DNA-Directed DNA Polymerase metabolism In Vitro Macromolecular Substances Nuclear Proteins pharmacology Poly dA-dT metabolism Proliferating Cell Nuclear Antigen RNA Nucleotidyltransferases metabolism Simian virus 40 genetics Templates Genetic Time Factors Virus Replication |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication organism description > virus |
| CSHL Authors: | |
| Communities: | CSHL labs > Stillman lab |
| Highlight: | Stillman, Bruce W. |
| Depositing User: | CSHL Librarian |
| Date: | 25 January 1991 |
| Date Deposited: | 28 Feb 2012 19:54 |
| Last Modified: | 20 Jun 2017 20:11 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25121 |
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