Tsurimoto, T., Stillman, B. (February 1990) Functions of replication factor C and proliferating-cell nuclear antigen: functional similarity of DNA polymerase accessory proteins from human cells and bacteriophage T4. Proceedings of the National Academy of Sciences of the United States of America, 87 (3). pp. 1023-7. ISSN 0027-8424
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Abstract
The proliferating-cell nuclear antigen (PCNA) and the replication factors A and C (RF-A and RF-C) are cellular proteins essential for complete elongation of DNA during synthesis from the simian virus 40 origin of DNA replication in vitro. All three cooperate to stimulate processive DNA synthesis by DNA polymerase delta on a primed single-stranded M13 template DNA and as such can be categorized as DNA polymerase accessory proteins. Biochemical analyses with highly purified RF-C and PCNA have demonstrated functions that are completely analogous to the functions of bacteriophage T4 DNA polymerase accessory proteins. A primer-template-specific DNA binding activity and a DNA-dependent ATPase activity copurified with the multisubunit protein RF-C and are similar to the functions of the phage T4 gene 44/62 protein complex. Furthermore, PCNA stimulated the RF-C ATPase activity and is, therefore, analogous to the phage T4 gene 45 protein, which stimulates the ATPase function of the gene 44/62 protein complex. Indeed, some primary sequence similarities between human PCNA and the phage T4 gene 45 protein could be detected. These results demonstrate a striking conservation of the DNA replication apparatus in human cells and bacteriophage T4.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Adenosinetriphosphatase isolation & purification metabolism Amino Acid Sequence Autoantigens Bacterial Proteins genetics metabolism Cell Line Cloning Molecular Comparative Study DNA Helicases metabolism DNA Replication DNA-Binding Proteins metabolism DNA-Directed DNA Polymerase metabolism Escherichia coli metabolism Humans Kinetics Molecular Sequence Data Nuclear Proteins genetics metabolism Proliferating Cell Nuclear Antigen Sequence Homology Nucleic Acid T-Phages metabolism Templates Genetic |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage |
| CSHL Authors: | |
| Communities: | CSHL labs > Stillman lab |
| Highlight: | Stillman, Bruce W. |
| Depositing User: | CSHL Librarian |
| Date: | February 1990 |
| Date Deposited: | 28 Feb 2012 19:47 |
| Last Modified: | 30 Sep 2019 16:34 |
| PMCID: | PMC53402 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25119 |
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