Cdc6p modulates the structure and DNA binding activity of the origin recognition complex in vitro

Mizushima, T., Takahashi, N., Stillman, B. (July 2000) Cdc6p modulates the structure and DNA binding activity of the origin recognition complex in vitro. Genes & Development, 14 (13). pp. 1631-1641. ISSN 0890-9369

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URL: http://www.ncbi.nlm.nih.gov/pubmed/10887157

DOI: 10.1101/gad.14.13.1631

Abstract

An interaction between the origin recognition complex (ORC) and Cdc6p is the first and a key step in the initiation of chromosomal DNA replication. We describe the assembly of an origin-dependent complex containing ORC and Cdc6p from Saccharomyces cerevisiae. Cdc6p increases the DNA binding specificity of ORC by inhibiting non-specific DNA binding of ORC. Cdc6p induces a concomitant change in the conformation of ORC and mutations in the Cdc6p Walker A and Walker B motifs, or ATP-gamma-S inhibited these activities of Cdc6p. These data suggest that Cdc6p modifies ORC function at DNA replication origins. On the basis of these results in yeast, we propose that Cdc6p may be an essential determinant of origin specificity in metazoan species.

Item Type:	Paper
Uncontrolled Keywords:	ORC Cdc6p ATPase sequence specificity DNA replication S. cerevisiae YEAST REPLICATION ORIGINS yeast replication origins CYCLIN-DEPENDENT KINASE cyclin dependent kinase S-PHASE S-Phase SACCHAROMYCES-CEREVISIAE Saccharomyces Cerevisiae BUDDING YEAST Budding yease MCM PROTEINS MCM Proteins FISSION YEAST Fission yease CELL-CYCLE cell cycle SCHIZOSACCHAROMYCES-POMBE Schizosaccharomyces Pombe G(1) PHASE Phase
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > origin recognition complex
CSHL Authors:	Stillman, Bruce W. Mizushima, Tohru
Communities:	CSHL labs > Stillman lab
Highlight:	Stillman, Bruce W.
Depositing User:	CSHL Librarian
Date:	July 2000
Date Deposited:	02 Mar 2012 19:55
Last Modified:	20 Jun 2017 18:51
PMCID:	PMC316732
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/25024

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