Liang, C., Stillman, B. (December 1997) Persistent initiation of DNA replication and chromatin-bound MCM proteins during the cell cycle in cdc6 mutants. Genes and Development, 11 (24). pp. 3375-86. ISSN 08909369 (ISSN)
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Abstract
Faithful inheritance of genetic information requires that DNA be copied only once each cell cycle. Initiation of DNA replication involves the establishment of a prereplication complex (pre-RC) and subsequent activation by CDK/cyclins, converting the pre-RC to a post-RC. The origin recognition complex (ORC), Cdc6p, and the MCM proteins are required for establishing the pre-RC. We show that all six ORC subunits remain bound to chromatin throughout the cell cycle, whereas the MCM proteins cycle on and off, corresponding precisely to transitions of the RC. A newly isolated cdc6 mutant displays promiscuous initiation of DNA replication, increased nuclear DNA content, and constant MCM protein association with chromatin throughout the cell cycle. This gain-of-function cdc6 mutant ignores the negative controls imposed normally on initiation by the CDK/cyclins, suggesting that Cdc6p is a key mediator of once-per-cell-cycle control of DNA replication.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | CDC28 Protein Kinase S cerevisiae metabolism Cell Cycle genetics Cell Cycle Proteins genetics/ metabolism Chromatin metabolism DNA Replication Fungal Proteins genetics/metabolism Mutation Replication Origin Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Temperature |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication |
| CSHL Authors: | |
| Communities: | CSHL labs > Stillman lab |
| Highlight: | Stillman, Bruce W. |
| Depositing User: | CSHL Librarian |
| Date: | 15 December 1997 |
| Date Deposited: | 06 Mar 2012 15:05 |
| Last Modified: | 20 Jun 2017 19:32 |
| PMCID: | PMC316796 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25005 |
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