Kaufman, P. D., Kobayashi, R., Kessler, N., Stillman, B. (June 1995) The p150 and p60 subunits of chromatin assembly factor I: a molecular link between newly synthesized histones and DNA replication. Cell, 81 (7). pp. 1105-14. ISSN 0092-8674 (Print)
Abstract
Chromatin assembly factor I (CAF-I) from human cell nuclei is a three-subunit protein complex that assembles histone octamers onto replicating DNA in a cell-free system. Sequences of cDNAs encoding the two largest CAF-I subunits reveal that the p150 protein contains large clusters of charged residues, whereas p60 contains WD repeats. p150 and p60 directly interact and are both required for DNA replication-dependent assembly of nucleosomes. Deletion of the p60-binding domain from the p150 protein prevents chromatin assembly. p150 and p60 form complexes with newly synthesized histones H3 and acetylated H4 in human cell extracts, suggesting that such complexes are intermediates between histone synthesis and assembly onto replicating DNA.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amino Acid Sequence Base Sequence Cell Line Cell Nucleus metabolism Chromosomal Proteins Non-Histone Cloning Molecular DNA Replication DNA-Binding Proteins biosynthesis isolation & purification metabolism Histones biosynthesis isolation & purification metabolism Humans Macromolecular Substances Molecular Sequence Data Oligodeoxyribonucleotides Protein Binding Protein Biosynthesis Recombinant Proteins biosynthesis isolation & purification metabolism Repetitive Sequences, Nucleic Acid Sequence Deletion |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication |
| CSHL Authors: | |
| Communities: | CSHL labs > Kobayashi lab CSHL labs > Stillman lab |
| Highlight: | Stillman, Bruce W. |
| Depositing User: | CSHL Librarian |
| Date: | 30 June 1995 |
| Date Deposited: | 06 Mar 2012 15:31 |
| Last Modified: | 20 Jun 2017 19:43 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/24994 |
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