Cell-cycle-regulated phosphorylation of DNA replication factor A from human and yeast cells

Din, S., Brill, S. J., Fairman, M. P., Stillman, B. (June 1990) Cell-cycle-regulated phosphorylation of DNA replication factor A from human and yeast cells. Genes Dev, 4 (6). pp. 968-77. ISSN 0890-9369

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Abstract

Replication factor A (RF-A) is a multisubunit, cellular protein that functions with SV40 T antigen during the initiation stage of DNA replication at the SV40 origin. It also cooperates with other replication factors to stimulate the activity of both polymerases alpha and delta during chain elongation. RF-A from both human and yeast cells is phosphorylated in a cell-cycle-dependent manner; the protein is phosphorylated at the G1- to S-phase transition, and dephosphorylation occurs at mitosis, thereby resetting this cycle. This observation provides a direct link between a protein required for DNA replication and cell-cycle-regulated protein phosphorylation.

Item Type:	Paper
Additional Information:
Uncontrolled Keywords:	DNA Replication DNA-Binding Proteins metabolism Hela Cells Humans Immunoblotting Interphase Mitosis Phosphorylation Precipitin Tests Saccharomyces cerevisiae genetics metabolism Tumor Cells Cultured
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression > phosphorylation
CSHL Authors:	Stillman, Bruce W.
Communities:	CSHL labs > Stillman lab
Highlight:	Stillman, Bruce W.
Depositing User:	CSHL Librarian
Date:	June 1990
Date Deposited:	06 Mar 2012 17:22
Last Modified:	20 Jun 2017 20:15
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/24963

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