Cullmann, G., Fien, K., Kobayashi, R., Stillman, B. (September 1995) Characterization of the five replication factor C genes of Saccharomyces cerevisiae. Molecular and Cellular Biology, 15 (9). pp. 4661-71. ISSN 0270-7306
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Abstract
Replication factor C (RFC) is a five-subunit DNA polymerase accessory protein that functions as a structure-specific, DNA-dependent ATPase. The ATPase function of RFC is activated by proliferating cell nuclear antigen. RFC was originally purified from human cells on the basis of its requirement for simian virus 40 DNA replication in vitro. A functionally homologous protein complex from Saccharomyces cerevisiae, called ScRFC, has been identified. Here we report the cloning, by either peptide sequencing or by sequence similarity to the human cDNAs, of the S. cerevisiae genes RFC1, RFC2, RFC3, RFC4, and RFC5. The amino acid sequences are highly similar to the sequences of the homologous human RFC 140-, 37-, 36-, 40-, and 38-kDa subunits, respectively, and also show amino acid sequence similarity to functionally homologous proteins from Escherichia coli and the phage T4 replication apparatus. All five subunits show conserved regions characteristic of ATP/GTP-binding proteins and also have a significant degree of similarity among each other. We have identified eight segments of conserved amino acid sequences that define a family of related proteins. Despite their high degree of sequence similarity, all five RFC genes are essential for cell proliferation in S. cerevisiae. RFC1 is identical to CDC44, a gene identified as a cell division cycle gene encoding a protein involved in DNA metabolism. CDC44/RFC1 is known to interact genetically with the gene encoding proliferating cell nuclear antigen, confirming previous biochemical evidence of their functional interaction in DNA replication.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amino Acid Sequence Base Sequence Cell Cycle Proteins genetics Cell Division genetics Cloning Molecular DNA-Binding Proteins genetics DNA-Directed DNA Polymerase genetics Evolution Genes Fungal genetics Genes Lethal genetics Homeodomain Proteins Humans Infant Newborn Molecular Sequence Data Mutagenesis Proto Oncogene Proteins c-bcl-2 Replication Factor C Repressor Proteins Restriction Mapping Saccharomyces cerevisiae genetics growth & development Saccharomyces cerevisiae Proteins Sequence Analysis DNA Sequence Homology Amino Acid Species Specificity |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > DNA polymerase bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication |
| CSHL Authors: | |
| Communities: | CSHL labs > Kobayashi lab CSHL labs > Stillman lab |
| Highlight: | Stillman, Bruce W. |
| Depositing User: | CSHL Librarian |
| Date: | September 1995 |
| Date Deposited: | 07 Mar 2012 16:03 |
| Last Modified: | 20 Jun 2017 19:43 |
| PMCID: | PMC230709 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/24950 |
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