Carpino, N., Wisniewski, D., Strife, A., Marshak, D., Kobayashi, R., Stillman, B., Clarkson, B. (January 1997) p62(dok): a constitutively tyrosine-phosphorylated, GAP-associated protein in chronic myelogenous leukemia progenitor cells. Cell, 88 (2). pp. 197-204. ISSN 0092-8674 (Print)
Abstract
Characteristic of chronic myelogenous leukemia (CML) is the presence of the chimeric p210(bcr-abl) protein possessing elevated protein tyrosine kinase activity relative to normal c-abl tyrosine kinase. Hematopoietic progenitors isolated from CML patients in the chronic phase contain a constitutively tyrosine-phosphorylated protein that migrates at 62 kDa by SDS-PAGE and associates with the p120 ras GTPase-activating protein (GAP). We have purified p62(dok) from a hematopoietic cell line expressing p210(bcr-abl). p62(dok) is a novel protein with features of a signaling molecule. Association of p62(dok) with GAP correlates with its tyrosine phosphorylation. p62(dok) is rapidly tyrosine-phosphorylated upon activation of the c-Kit receptor, implicating it as a component of a signal transduction pathway downstream of receptor tyrosine kinases.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Cloning, Molecular DNA-Binding Proteins Electrophoresis Polyacrylamide Gel Fusion Proteins bcr-abl metabolism GTPase-Activating Proteins Hematopoietic Stem Cells metabolism Humans Leukemia, Myeloid, Chronic metabolism Phosphoproteins chemistry isolation & purification metabolism Phosphorylation Phosphotyrosine metabolism Protein-Tyrosine Kinase metabolism Proteins metabolism Proto-Oncogene Proteins c-kit metabolism RNA-Binding Proteins Signal Transduction Stem Cell Factor metabolism Tumor Cells, Cultured ras GTPase-Activating Proteins src Homology Domains |
| Subjects: | diseases & disorders > cancer > cancer types > leukemia biotechnology > chromatography > protein purification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase > tyrosine kinase |
| CSHL Authors: | |
| Communities: | CSHL labs > Kobayashi lab CSHL labs > Stillman lab |
| Highlight: | Stillman, Bruce W. |
| Depositing User: | CSHL Librarian |
| Date: | 24 January 1997 |
| Date Deposited: | 07 Mar 2012 18:36 |
| Last Modified: | 20 Jun 2017 19:35 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/24946 |
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