Brill, S. J., Stillman, B. (September 1991) Replication factor-A from Saccharomyces cerevisiae is encoded by three essential genes coordinately expressed at S phase. Genes and Development, 5 (9). pp. 1589-600. ISSN 08909369 (ISSN)
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Abstract
Replication factor-A (RF-A) is a three-subunit protein complex originally purified from human cells as an essential component for SV40 DNA replication in vitro. We have previously identified a functionally homologous three-subunit protein complex from the yeast Saccharomyces cerevisiae. Here we report the cloning and characterization of the genes encoding RF-A from S. cerevisiae. Each of the three subunits is encoded by a single essential gene. Cells carrying null mutations in any of the three genes arrest as budded and multiply budded cells. All three genes are expressed in a cell-cycle-dependent manner; the mRNA for each subunit peaks at the G1/S-phase boundary. A comparison of protein sequences indicates that the human p34 subunit is 29% identical to the corresponding RFA2 gene product. However, expression of the human protein fails to rescue the rfa2::TRP1 disruption.
| Item Type: | Paper |
|---|---|
| Additional Information: | |
| Uncontrolled Keywords: | Amino Acid Sequence Base Sequence Cloning Molecular DNA Replication genetics DNA, Fungal DNA Binding Proteins genetics Fungal Proteins genetics Gene Expression Regulation Fungal Genes Fungal Humans Introns Molecular Sequence Data Phenotype Plasmids Restriction Mapping S Phase Saccharomyces cerevisiae genetics growth & development Sequence Alignment |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication |
| CSHL Authors: | |
| Communities: | CSHL labs > Stillman lab |
| Highlight: | Stillman, Bruce W. |
| Depositing User: | CSHL Librarian |
| Date: | September 1991 |
| Date Deposited: | 07 Mar 2012 18:24 |
| Last Modified: | 20 Jun 2017 20:09 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/24942 |
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