Bell, S. P., Kobayashi, R., Stillman, B. (December 1993) Yeast origin recognition complex functions in transcription silencing and DNA replication. Science, 262 (5141). pp. 1844-9. ISSN 0036-8075 (Print)
Abstract
The genes encoding two of the subunits of the Saccharomyces cerevisiae origin recognition complex (ORC) have been isolated. Characterization of a temperature-sensitive mutation in the gene encoding the 72-kD subunit of ORC (ORC2) indicates that this protein complex functions early in the DNA replication process. Moreover, ORC derived from orc2ts cells is defective for DNA binding. Others have shown a defect in orc2ts cells in transcriptional silencing at the silent mating-type loci. Consistent with this finding, ORC specifically binds to each of the four mating-type silencers identified in yeast. These findings support the hypothesis that ORC acts as an initiator protein at yeast origins of DNA replication and suggest that ORC also functions in the determination of transcriptional domains.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amino Acid Sequence DNA Replication DNA, Fungal biosynthesis DNA-Binding Proteins Fungal Proteins chemistry genetics/metabolism Gene Expression Regulation Fungal Genes, Fungal Genes, Mating Type Fungal Molecular Sequence Data Mutation Origin Recognition Complex Replicon Repressor Proteins chemistry genetics metabolism S Phase Saccharomyces cerevisiae cytology genetics/metabolism Saccharomyces cerevisiae Proteins Temperature Transcription Genetic |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > origin recognition complex |
| CSHL Authors: | |
| Communities: | CSHL labs > Stillman lab |
| Highlight: | Stillman, Bruce W. |
| Depositing User: | CSHL Librarian |
| Date: | 17 December 1993 |
| Date Deposited: | 07 Mar 2012 19:06 |
| Last Modified: | 20 Jun 2017 19:54 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/24935 |
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