Activity-dependent plasticity of the NMDA-receptor fractional Ca2+ current

Sobczyk, A., Svoboda, K. (January 2007) Activity-dependent plasticity of the NMDA-receptor fractional Ca2+ current. Neuron, 53 (1). pp. 17-24. ISSN 0896-6273 (Print)

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DOI: 10.1016/j.neuron.2006.11.016


Ca(2+) influx through NMDA receptors (NMDA-Rs) triggers synaptic plasticity, gene transcription, and cytotoxicity, but little is known about the regulation of NMDA-Rs themselves. We used two-photon glutamate uncaging to activate NMDA-Rs on individual dendritic spines in rat CA1 neurons while we measured NMDA-R currents at the soma and [Ca(2+)] changes in spines. Low-frequency uncaging trains induced Ca(2+)-dependent long-term depression of NMDA-R-mediated synaptic currents. Additionally, uncaging trains caused a reduction in the Ca(2+) accumulation per unit of NMDA-R current in spines due to a reduction in the fraction of the NMDA-R current carried by Ca(2+). Induction of depression of NMDA-R-mediated Ca(2+) influx required activation of NR2B-containing receptors. Receptors in single spines depressed rapidly in an all-or-none manner. These adaptive changes in NMDA-R function likely play a critical role in metaplasticity and in stabilizing activity levels in neuronal networks with Hebbian synapses.

Item Type: Paper
Uncontrolled Keywords: Animals Calcium/metabolism Calcium Signaling/drug effects/ physiology Dendritic Spines/drug effects/ metabolism/ultrastructure Glutamic Acid/metabolism/pharmacology Hippocampus/drug effects/ metabolism/ultrastructure Long-Term Depression (Physiology)/drug effects/physiology Nerve Net/drug effects/metabolism/ultrastructure Neural Pathways/drug effects/metabolism/ultrastructure Neuronal Plasticity/drug effects/ physiology Organ Culture Techniques Patch-Clamp Techniques Pyramidal Cells/drug effects/metabolism/ultrastructure Rats Rats, Sprague-Dawley Receptors, N-Methyl-D-Aspartate/drug effects/ metabolism Synaptic Transmission/drug effects/ physiology
Subjects: diseases & disorders > mental disorders > delirium dementia cognitive disorders > Alzheimer's disease
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > NMDA receptor
organism description > animal > mammal > rodent > rat
organism description > animal > mammal > rodent > rat
CSHL Authors:
Communities: CSHL labs > Svoboda lab
Depositing User: CSHL Librarian
Date: 4 January 2007
Date Deposited: 02 Nov 2011 16:33
Last Modified: 10 Apr 2018 16:25
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