Petreanu, L. T., Huber, D., Sobczyk, A., Svoboda, K. (May 2007) Channelrhodopsin-2-assisted circuit mapping of long-range callosal projections. Nat Neurosci, 10 (5). pp. 663-668. ISSN 1097-6256
Abstract
The functions of cortical areas depend on their inputs and outputs, but the detailed circuits made by long- range projections are unknown. We show that the light- gated channel channelrhodopsin- 2 ( ChR2) is delivered to axons in pyramidal neurons in vivo. In brain slices from ChR2- expressing mice, photostimulation of ChR2- positive axons can be transduced reliably into single action potentials. Combining photostimulation with whole- cell recordings of synaptic currents makes it possible to map circuits between presynaptic neurons, defined by ChR2 expression, and postsynaptic neurons, defined by targeted patching. We applied this technique, ChR2- assisted circuit mapping ( CRACM), to map long- range callosal projections from layer ( L) 2/3 of the somatosensory cortex. L2/3 axons connect with neurons in L5, L2/3 and L6, but not L4, in both ipsilateral and contralateral cortex. In both hemispheres the L2/3- to- L5 projection is stronger than the L2/3- to- L2/3 projection. Our results suggest that laminar specificity may be identical for local and longrange cortical projections.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | VISUAL-CORTEX SYNAPTIC CONNECTIONS SOMATOSENSORY CORTEX FLUORESCENT PROTEIN CORTICAL CIRCUITS CORPUS-CALLOSUM OPTICAL CONTROL RAT NEOCORTEX ADULT-RAT NEURONS |
| Subjects: | organs, tissues, organelles, cell types and functions > tissues types and functions > cerebral cortex bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > exons organism description > animal > mammal > rodent > mouse |
| CSHL Authors: | |
| Communities: | CSHL labs > Svoboda lab |
| Depositing User: | CSHL Librarian |
| Date: | May 2007 |
| Date Deposited: | 07 Nov 2011 20:56 |
| Last Modified: | 02 Apr 2018 20:28 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/23119 |
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