Ehrlich, I., Klein, M. E., Rumpel, S., Malinow, R. (March 2007) PSD-95 is required for activity-driven synapse stabilization. Proc Natl Acad Sci U S A, 104 (10). pp. 4176-81. ISSN 0027-8424 (Print)
Preview |
PDF (Paper)
PSD-95 is required.pdf - Published Version Download (1MB) | Preview |
Abstract
The activity-dependent regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors and the stabilization of synapses are critical to synaptic development and plasticity. One candidate molecule implicated in maturation, synaptic strengthening, and plasticity is PSD-95. Here we find that acute knockdown of PSD-95 in brain slice cultures by RNAi arrests the normal development of synaptic structure and function that is driven by spontaneous activity. Surprisingly, PSD-95 is not necessary for the induction and early expression of long-term potentiation (LTP). However, knockdown of PSD-95 leads to smaller increases in spine size after chemically induced LTP. Furthermore, although at this age spine turnover is normally low and LTP produces a transient increase, in cells with reduced PSD-95 spine turnover is high and remains increased after LTP. Taken together, our data support a model in which appropriate levels of PSD-95 are required for activity-dependent synapse stabilization after initial phases of synaptic potentiation.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > PSD95 bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > glutamate receptor organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > synapse |
| CSHL Authors: | |
| Communities: | CSHL labs > Malinow lab |
| Depositing User: | CSHL Librarian |
| Date: | 6 March 2007 |
| Date Deposited: | 15 Nov 2011 16:45 |
| Last Modified: | 22 Mar 2018 20:54 |
| PMCID: | PMC1820728 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/23008 |
Actions (login required)
 |
Administrator's edit/view item |