Bannigan, A., Scheible, W. R., Lukowitz, W., Fagerstrom, C., Wadsworth, P., Somerville, C., Baskin, T. I. (August 2007) A conserved role for kinesin-5 in plant mitosis. Journal of Cell Science, 120 (16). pp. 2819-2827. ISSN 0021-9533
Abstract
The mitotic spindle of vascular plants is assembled and maintained by processes that remain poorly explored at a molecular level. Here, we report that AtKRP125c, one of four kinesin-5 motor proteins in arabidopsis, decorates microtubules throughout the cell cycle and appears to function in both interphase and mitosis. In a temperature-sensitive mutant, interphase cortical microtubules are disorganized at the restrictive temperature and mitotic spindles are massively disrupted, consistent with a defect in the stabilization of anti-parallel microtubules in the spindle midzone, as previously described in kinesin-5 mutants from animals and yeast. AtKRP125c introduced into mammalian epithelial cells by transfection decorates microtubules throughout the cell cycle but is unable to complement the loss of the endogenous kinesin-5 motor (Eg5). These results are among the first reports of any motor with a major role in anastral spindle structure in plants and demonstrate that the conservation of kinesin-5 motor function throughout eukaryotes extends to vascular plants.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Arabidopsis thaliana AtKRP125 cortical microtubules Eg5 gamma-tubulin root morphology CROSS-LINKS Microtubules CULTURED TOBACCO CELLS mitotic spindle GAMMA-TUBULIN CORTICAL MICROTUBULES CYTOKINETIC APPARATUS STABLE EXPRESSION MAMMALIAN-CELLS SMALL-MOLECULE CARROT CELLS |
| Subjects: | organism description > plant > Arabidopsis bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > kinesin-5 protein organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > microtubule organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > mitotic spindle organism description > plant |
| CSHL Authors: | |
| Communities: | CSHL labs > Lukowitz lab |
| Depositing User: | CSHL Librarian |
| Date: | August 2007 |
| Date Deposited: | 02 Dec 2011 17:25 |
| Last Modified: | 21 Mar 2018 14:31 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/22966 |
Actions (login required)
 |
Administrator's edit/view item |